Beautiful blue glow arises during the chemical reaction
name: Chemiluminescence of luminol oxidation occurs as a result of oxidation
Bioluminescence Bleach and Luminol Oxidation - MaxTurner91
by Winston Turner
Luminol Reaction - Organic Chemistry
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C8H7N3O2 + 2 OH(-) + O2 → C8H5NO4(2-) + hv
- Prepare the first solution. To do this, take 100 ml of bleach (5% sodium hypochlorite solution) and 900 ml of water, put the ingredients into the flask and stir the components vigorously. The first solution is prepared.
- Prepare the second solution. This would require 0.4 g of luminol and 4 g of sodium hydroxide and 1 liter of water. Dissolve the listed components in water and mix it.
- Turn off the lights in the room.
- Slowly pour the first solution to the second solution with stirring.
- Observe the blue glow.
- Other variations of excitation the luminescence of luminol are possible with other oxidizers (e.g., hydrogen peroxide). For details, see. Links.
Luminol forms dianions when it interact with hydroxide ions, which react with oxygen which is ensured by the presence of an oxidant. The product of this reaction is a highly unstable organic biradical that instantly dissolves to form a molecule of nitrogen and 3-aminophthalic acid in the excited electronic state. When the molecule returns from the excited to the ground electronic state a photon is released.