Column Chromatography

Separation of substances in the column

Scientific name: The substances pass through the column at different rates. First, the concentration of exhaust substances increases and then decreases.


Column Chromatography

by David Read

Column Chromatography

by ButteScience

Column Chromatography

by khanacademymedicine

Column Chromatography

by S-Cubed Malta

Column chromatography demonstration

by medchem90

Column Chromatography | MIT Digital Lab Techniques Manual

by MIT OpenCourseWare

Isolation of Plant Pigments by Column Chromatography - Amrita University

by Amrita Vlab


Wear eye protection goggles.

Always follow general safety recommendations. Please note that conducting chemistry experiments you must comply with the relevant legal procedures in your country.

Step-by-step instruction

  1. Take a piece of cotton wool and insert into the coloumn (towards outlet).
  2. Fix the column to the clamp.
  3. Pour in a small bed of sea sand.
  4. Add enough solvent (usually lower polar solvent).
  5. Pour silica slurry slowly into the column while stirring.
  6. Rinse the column wall to remove adsorbed silica.
  7. Mark the silica level and gently tap with cork rings (or hand or soft materials)
  8. Dissolve the sample and put a little silica. Dry it with a rotary evaporator.
  9. Pour dry sample slowly and rinse the wall.
  10. Put a little sand on top of the sample.
  11. Collect the fractions.

Scientific background

Separation of multicomponent mixtures into a series of binary mixtures with a liquid and one of the separating components takes place in a chromatography column. Separation can occur due to the formation of temporary bonds between the separating substances and the stationary phase of column (adsorption chromatography), or due to other principles (e.g. size exclusion chromatography).


Published on 13 June 2015

  • Fire
  • Heating with fire
  • Explosion
  • Poisoned gas
  • Organic
  • Electricity
  • Solution
  • Oxidation reduction
  • Color change
  • Precipitate
  • Gassing
  • Catalyst