Spots of the substances move upwards with different speeds by immersing the plate in a solvent
name: Substances are differently distributed between the absorbent layer and the eluent flowing through it, whereby the distances of shifting at the same time of these substances are different
Thin-layer chromatography is a chromatographic method based on the use of a thin layer of adsorbent as the stationary phase. It is based on the fact that the separated substances are distributed differently between the sorbent and eluent flowing through it, whereby the distances of shifting at the same time of these substances are different. TLC provides great opportunities for analysis and separation of substances, as adsorbent and the eluent can vary within wide limits. Series of plates is commercially available with various sorbents, it makes it possible to quickly and routinely use the method. There are more reliable and reproducible kind of TLC, like high-performance thin-layer chromatography (HPLC), which uses special plates and sophisticated equipment.
TLC was established in 1889 and essentially developed from the middle of the XX century to the present time and is widely used in pharmaceutical, medical and food sectors, as well as in the academic and industrial science.